Part:BBa_K1618046

THL.BHBD.CRO.BCD.EFb.EFa.BUK.PTB

K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate ¹. At the last moment, we realised that there was an EcoRI site on the gene sequence, we're working to fix it as soon as possible.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 229
  Illegal suffix found in sequence at 7178
  Illegal EcoRI site found at 3159
  Illegal EcoRI site found at 7207
  Illegal XbaI site found at 7222
  Illegal SpeI site found at 8122
  Illegal PstI site found at 8136
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 229
  Illegal EcoRI site found at 3159
  Illegal EcoRI site found at 7207
  Illegal NheI site found at 1167
  Illegal SpeI site found at 7179
  Illegal SpeI site found at 8122
  Illegal PstI site found at 7193
  Illegal PstI site found at 8136
  Illegal NotI site found at 235
  Illegal NotI site found at 3841
  Illegal NotI site found at 7186
  Illegal NotI site found at 7213
  Illegal NotI site found at 8129
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 229
  Illegal EcoRI site found at 3159
  Illegal EcoRI site found at 7207
  Illegal BglII site found at 1415
  Illegal BglII site found at 6388
  Illegal BglII site found at 6628
  Illegal BglII site found at 6769
  Illegal BglII site found at 7546
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 229
  Illegal suffix found in sequence at 7179
  Illegal EcoRI site found at 3159
  Illegal EcoRI site found at 7207
  Illegal XbaI site found at 7222
  Illegal SpeI site found at 8122
  Illegal PstI site found at 8136
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 229
  Illegal EcoRI site found at 3159
  Illegal EcoRI site found at 7207
  Illegal XbaI site found at 244
  Illegal XbaI site found at 7222
  Illegal SpeI site found at 7179
  Illegal SpeI site found at 8122
  Illegal PstI site found at 7193
  Illegal PstI site found at 8136
  Illegal AgeI site found at 1338
  Illegal AgeI site found at 3354
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 7372